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Abstract: The selectivity of gamma-oryzanol ( γ-oryzanol) was recognized by molecularly imprinted polymer (MIP). Polymeric materials were successfully synthesized via thermal polymerization method using γ-oryzanol as template, anacardic acid (AnAc) as functional monomer, toluene as porogen, benzoyl peroxide (BPO) as initiator and divinylbenzene as crosslinker. Binding performance of MIPs was evaluated by MINITAP 14 for variance of analysis, linear regression analysis and adequating model through full factorial experimental technique in terms of adsorption capacity. Analysis of variance with 95% confidence level suggested significant interaction effect (amounts of template, porogen, crosslinker) on adsorption capacity of MIPs. The strongest interaction is between the amount of porogen and the amount of crosslinker. It was also found that a linear regression model for adsorption capacity represents the experimental data with the correlation coefficients (R 2 ) greater than 0.9. The MIP synthesis with 0.8 mmol of template, 6 ml of porogen and 10 ml of crosslinker provided the highest adsorption capacity of MIP (1.14 mg/g-adsorbent). The proposed method is relatively rapid and easy to perform for the separation of γ-oryzanol in non-aqueous systems
Template and target information: γ-oryzanol
Author keywords: Analysis of Variance, molecularly imprinted polymer, molecular recognition, Gamma-oryzanol, anacardic acid