In this work, the nanoparticles of promethazine-imprinted polymers (MIP) were synthesized by the ultrasonic assisted suspension polymerization in silicon oil. The MIP particles were then embedded in a carbon paste (CP) electrode in order to prepare the MIP (nano)-CP electrode. This electrode showed higher response to analyte, compared to the carbon paste electrode, modified with non-imprinted polymer (NIP (nano)-CP). Also, the sensitivity and selectivity of the MIP (nano)-CP were considerably better than those of the sensor prepared by the bulky MIP particles (MIP (micro)-CP). Various factors, known to affect the response behavior of selective electrode, were investigated and optimized. The sensor exhibited two distinct linear response ranges of 4 x 10-12 - 1 x 10-10 M and 1 x 10-9 - 1 x 10-7 M with the sensitivities of 31.7 and 0.17 μA nM-1, respectively. The lower detection limit of the sensor was calculated equal to 2.8 x 10-12 M (S/N). An interestingly low RSD equal to 0.81% was found for 5 separate determinations by the proposed sensor. The sensor was applied for promethazine (PMZ) determination in plasma samples without applying any sample pretreatment.
Template and target information: promethazine, PMZ
Author keywords: Ultratrace, molecularly imprinted polymer, nanoparticles, suspension polymerization, silicon oil, Promethazine