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Reference type: Journal
Authors: Li JP, Li SH, Wei XP, Tao HL, Pan HC
Article Title: Molecularly Imprinted Electrochemical Luminescence Sensor Based On Signal Amplification for Selective Determination of Trace Gibberellin A3.
Publication date: 2012
Journal: Analytical Chemistry
Volume: 84
Issue: (22)
Page numbers: 9951-9955.
DOI: 10.1021/ac302401s

Abstract: A new molecularly imprinted electrochemical luminescence (MIP-ECL) sensor was developed for Gibberellin A3 (GA3) determination. This sensor is based on competitive binding between the GA3 and the Rhodamine B (RhB)-labeled GA3 (RhB-GA3) to the MIP film. After the competitive binding, the residual RhB-GA3 on the MIP was electro-oxidized to produce RhB oxide, which could greatly amplify the weak electrochemiluminescence (ECL) signal of luminol. The ECL intensity decreased when the RhB-GA3 was replaced by GA3 molecules in the samples. Accordingly, GA3 was determined in the concentration range from 1 x 10-11 to 3 x 10-9 mol/L with a detection limit of 3.45 x 10-12 mol/L. The sensor shows high sensitivity and selectivity, wide response range, good accuracy, and fast response. Beer samples were assayed by using the sensors, and the recoveries ranging from 96.0% to 103.2% were obtained
Template and target information: gibberellin A3, GA3


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