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Reference type: Journal
Authors: Latif U, Dickert FL
Article Title: Biomimetic Materials for Enrichment of Bioanalytes-Sensor Applications.
Publication date: 2012
Journal: Journal of Biolgical Research
Volume: 1
Issue: (3)
Page numbers: 63-68.
DOI: j.issn.2222-3203.2012.01.037

Abstract: The combination of a highly sensitive and selective recognition layers with a transducer leads to fabricate sensor devices for the detection of analytes over a wide range of sizes from few nanometers upto micrometers. Employing natural anti-sesame antibodies on quartz crystal microbalance produces significantly higher sensor responses towards sesame protein in comparison to brazilnut protein. Afterwards, artificial antibodies as receptors are produced via in situ molecular imprinting leads to further increase in sensitivity while comparing to natural ones. This strategy can also applied to more complex bioanalytes such as viruses in which structural features of tobacco mosaic virus are transferred to polyacrylates and polyurethane by following imprinting approach to detect the templated bioanalyte. Variation of template concentration in polyurethane imprints plays an important role in determining the interaction behavior of sensitive layer. Molecular imprinting technique also helps us to synthesize artificial receptors for larger particles such as yeast and bacteria cells which lead to detect growth stages of yeast cells as well as can differentiate between bacteria cell strains. Molecular imprinting technique also helps us to design an artificial recognition material for detecting plant pollen. The thickness of the sensitive layer plays an important role for reversible inclusion of templated analytes. Moreover, measurements depict that sizes and interactions between templated analytes and hollows determine the Sauerbrey and non- Sauerbrey behavior of sensor devices.
Template and target information: protein, viruses, pollen, sesame protein, tobacco mosaic virus, TMV
Author keywords: molecular imprinting, artificial antibodies, quartz crystal microbalance (QCM), proteins, Viruses, cells, Pollen

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