Reference type: Journal
Authors: Xiao DL, Dramou P, Xiong NQ, He H, Li H, Yuan DH, Dai H
Article Title: Development of novel molecularly imprinted magnetic solid-phase extraction materials based on magnetic carbon nanotubes and their application for the determination of gatifloxacin in serum samples coupled with HPLC.
Publication date: 2013
Journal: Journal of Chromatography A
Volume: 1274
Page numbers: 44-53.
DOI: 10.1016/j.chroma.2012.12.011

Abstract: A novel composite imprinted material, on the basis of magnetic carbon nanotubes (MCNTs)-incorporated layer using gatifloxacin as a template, methacrylic acid as a functional monomer, and ethylene glycol dimethacrylate as a cross-linker, was successfully synthesized by a surface imprinting technique. Adsorption dynamics and a Scatchard adsorption model were employed to evaluate the adsorption process. The results showed that magnetic carbon nanotubes molecularly imprinted polymers (MCNTs@MIP) displayed a rapid dynamic adsorption and a high adsorption capacity of 192.7 μg/mg towards GTFX. Applied MCNTs@MIP as a sorbent, a magnetic solid phase extraction method coupled with high performance liquid chromatography (MSPE-HPLC) was developed for determination of GTFX in serum samples. The recoveries from 79.1% ± 4.8% to 85.3% ± 4.2% were obtained. MCNTs@MIP can not only be collected and separated fast by external magnetic field but also have high surface-to-volume ratio, outstanding mechanical properties and specific recognition towards template molecule. In addition, The MCNTs@MIP could be regenerated, which could be used for five cycles with lost of less than 7.8% of its recovery on average. These analytical results of serum samples display that the proposed method based on MCNTs@MIP is applicable for fast and selective extraction of therapeutic agents from biological fluids
Template and target information: gatifloxacin, GTFX
Author keywords: Molecularly imprinted polymers, Magnetic carbon nanotubes, Magnetic solid phase extraction, Gatifloxacin, serum samples