MIPs logo MIPdatabase      Use this space
Custom Search
Reference type: Journal
Authors: Krupadam RJ, Venkatesh A, Piletsky SA
Article Title: Molecularly Imprinted Polymer Receptors for Nicotine Recognition in Biological systems.
Publication date: 2013
Journal: Molecular Imprinting
Volume: 1
Issue: (1)
Page numbers: 27-34.
DOI: 10.2478/molim-2012-0004

Abstract: Molecular imprinting of nicotine and related carcinogenic chemicals in itaconic acid ethylene glycol dimethacrylate copolymer is described. Molecularly imprinted polymers (MIPs) are made to contain binding sites capable of recognizing nicotine; thus the fingerprint of the nicotine created in the polymer allows it to serve as an ideal molecular recognition element. We demonstrate that the imprinted polymers show high selective binding affinity in biological buffers. This is a previously un-described initiative for molecular imprinting, since the binding occurs under conditions relevant to biological systems. Due to effect of molecular imprinting nanocavities with size 245 nm were formed and these nicotine receptor sites were distributed homogeneously in the nicotine imprinted polymer. The nicotine receptors showed highly selective to nicotine with Kd values as low as 10-5M, and the levels of selectivity similar to those of natural molecules - acetylcholine esterase (AChE). The recognition properties of the polymer receptor were analyzed using ultra-violet spectroscopy, computer simulations and adsorption assay. Importantly, the receptors were effective in wide pH range (6.8-8.2) while the natural nicotine receptors showed high binding only at pH 7.6. The high specificity and stability of artificial receptors rendered them promising alternatives to enzymes, antibodies, and other natural receptors useful in biomedical assays, sensors and drug development.
Template and target information: nicotine
Author keywords: molecular imprinting, molecular modeling, nicotine receptors, Polymer receptors, Selective binding


  Shirt featuring the name Sergey spelled out in symbols of the chemical elements  Periodic table Bag bag  Meerkat somebody mention coffee mug

Molecules Special Issue call      Appeal for information






 

Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:



Mickey Mouse 90th Anniversary banner