Abstract: Objective A series of molecularly imprinted polymers (MIPs) were prepared by precipitation polymerization and coupled with high performance liquid chromatography for determination of trace melamine in milk powder.
Methods MIPs were prepared using melamine and the conditions of polymerization, including porogen, cross-linker, the molar ratio of functional monomer and template, were studied to obtain the optimized MIPs with specific recognition ability and high adsorption capacity. Subsequently, it was used as the sorbents of solid phase extraction (SPE) to purify and enrich 60 mL extracted solution.
Results The recovery was changed from 85.1% to 99.7%. The molecular imprinting solid phase extraction procedure not only avoided the tedious procedure of sample preconcentration and shortened the analytical time, but also reduced the opportunity of pollution. Compared with MCX column recommended by national standard method (GB/T 22388-2008), purification effect and recovery of the MIPs column were more satisfactory. The spiked recovery of this method was changed from 89.7%—100.6% and the RSD was lower than 3.1%.
Conclusion A simple and sensitive method using MIPs as the sorbents of SPE was applied to determine trace melamine in milk powder, which could meet the needs of the routine testing work.
Template and target information: melamine
Author keywords: Molecularly imprinted polymers, large volume extraction, Melamine, Milk powder