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Reference type: Journal
Authors: Wang ZH, Li JS, Liu XL, Yang JM, Lu XQ
Article Title: Preparation of an amperometric sensor for norfloxacin based on molecularly imprinted grafting photopolymerization.
Publication date: 2013
Journal: Analytical and Bioanalytical Chemistry
Volume: 405
Issue: (8)
Page numbers: 2525-2533.
DOI: 10.1007/s00216-012-6678-z

Abstract: A sensitive amperometric sensor for norfloxacin (NF) was introduced. The receptor layer was prepared by molecularly imprinted photopolymerization of acrylamide and trimethylolpropane trimethacrylate on the surface of a gold electrode. The binding mechanism of the molecularly imprinted polymer was explored by ultraviolet (UV) and infrared (IR) spectroscopy. The chemosensor was characterized by cyclic voltammetry (CV), differential pulse voltammetry (DPV), electrochemical impedance (EI), and scanning electron microscopy (SEM). The electrode prepared by photopolymerization has a better recognition ability to template molecules than that of electropolymerization and NIP. Some parameters affecting sensor response were optimized. Norfloxacin was detected by measurements of an amperometric iūt curve. The linear relationships between current and logarithmic concentration are obtained from 1.0 x 10-9 to 1.0 x 10-3 mol L-1. The detection limit of the sensor was 1.0 x 10-10 mol L-1. The proposed method is sensitive, simple, and cheap, and is applied to detect NF in human urine successfully
Template and target information: norfloxacin, NF
Author keywords: molecularly imprinted polymer, norfloxacin, photopolymerization, sensor


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