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Reference type: Journal
Authors: Euterpio MA, Pagano I, Piccinelli AL, Rastrelli L, Crescenzi C
Article Title: Development and Validation of a Method for the Determination of (E)-Resveratrol and Related Phenolic Compounds in Beverages Using Molecularly Imprinted Solid Phase Extraction.
Publication date: 2012
Journal: Journal of Agricultural and Food Chemistry
Volume: 61
Issue: (8)
Page numbers: 1640-1645.
DOI: 10.1021/jf303251m

Abstract: A molecularly imprinted polymer was prepared using (E)-resveratrol as template and was evaluated for multicomponent multiclass analysis of polyphenolic compounds in complex matrices such as natural and alcoholic beverages. Chromatographic evaluation of the polymer exhibited high selectivity for (E)-resveratrol and its structural analogues, quercetin, and other flavonoids. An analytical procedure based on molecularly imprinted solid phase extraction (MISPE) and high-performance liquid chromatography coupled to UV detector was developed and validated for determination of (E)-resveratrol and quercetin in wine and fruit juice samples. The specific binding capacity of the MIP was estimated as 80 μg g-1 polymer by the cartridge test. MISPE sample pretreatment allows an excellent sample cleanup, enormously decreasing the number of coextracted potentially interfering compounds. Under the described conditions, by extracting 2 mL samples a clean extract is obtained and (E)-resveratrol and quercetin could be easily identified at concentration levels of, respectively, 1.5 and 7.0 μg L-1
Template and target information: (E)-Resveratrol, quercetin, flavonoids
Author keywords: molecularly imprinted polymer, solid phase extraction, flavonoids, (E)-resveratrol, quercetin, wine, red fruits juice, lingonberry


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