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Reference type: Journal
Authors: Du W, Fu Q, Zhao G, Huang P, Jiao YY, Wu H, Luo ZM, Chang C
Article Title: Dummy-template molecularly imprinted solid phase extraction for selective analysis of ractopamine in pork.
Publication date: 2013
Journal: Food Chemistry
Volume: 139
Issue: (1-4)
Page numbers: 24-30.
DOI: 10.1016/j.foodchem.2013.01.109
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0308814613001453

Abstract: Molecularly imprinted polymers (MIPs) for selective adsorption of ractopamine hydrochloride (RAC) were synthesised by an in situ method, in which salbutamol (SAL) was used as the dummy-template to avoid the template leakage. Scanning electron microscopy (SEM), mercury porosimerty and Fourier transform infrared spectroscopy (FTIR) were used to investigate the physical and morphological characteristics of the dummy-template MIPs. The test of adsorption selectivity indicated that the dummy-template MIPs exhibited high selectivity to RAC. The saturated adsorption capacity for RAC on dummy-template MIPs was 90.9 μg g-1. Based on the dummy-template polymers, a liquid chromatography-mass spectrometry (LC-MS) method was developed for the selective analysis of RAC in real pork samples. The averages of intra- and inter-day accuracy ranged from 78.9% to 92.2% and from 90.7% to 93.1%, respectively. The RSD% of repeatability ranged from 1.9% to 6.3%, and the RSD% of intermediate precision ranged from 3.5% to 9.2%, while the limit of detection (LOD) was 0.02 μg kg-1
Template and target information: salbutamol, SAL, ractopamine, RAC, dummy template
Author keywords: Ractopamine, Dummy-template, molecularly imprinting polymers, In situ polymerisation, solid phase extraction, liquid chromatography-mass spectrometry


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