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Reference type: Journal
Authors: Çavus A, Baysal Z, Alkan H
Article Title: Preparation of poly(hydroxyethyl methacrylate) cryogels containing l-histidine for insulin recognition.
Publication date: 2013
Journal: Colloids and Surfaces B: Biointerfaces
Volume: 107
Page numbers: 84-89.
DOI: 10.1016/j.colsurfb.2013.01.075
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0927776513000994

Abstract: In the present study, affinity adsorption technique was studied for insulin adsorption. Firstly, insulin-imprinted supermacroporous cryogel was prepared for the insulin adsorption. N-methacryloyl-(l)-histidine methyl ester (MAH) was chosen as the monomer. Insulin was complexed with MAH, and insulin-imprinted p(HEMA-MAH) [insulin-(MIP)] cryogel was prepared by free radical polymerization with 2-hydroxyethyl methacrylate (HEMA), N,N,N',N'-tetramethylethylenediamine (TEMED) and ammonium persulfate (APS) in an ice bath. Then, insulin was removed from the cryogel by using 0.1 M glycine-HCl buffer (pH: 3.5). The characterization of the cryogel was carried out by using scanning electron microscopy (SEM) and swelling test. The equilibrium swelling ratios of the cryogels were found to be 8.56 ± 0.42 g H2O/g polymer for p(HEMA) and 7.20 ± 0.36 g H2O/g polymer for insulin-p(HEMA-MAH). Insulin adsorption experiments were performed under different conditions, such as flow rate, medium pH, initial insulin concentration and ionic strength. It was observed that insulin could be repeatedly adsorbed and desorbed with MIP cryogel without any significant decrease in the adsorption capacity
Template and target information: peptide, insulin
Author keywords: Insulin, affinity adsorption, molecular recognition, molecular imprinting, Cryogel


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