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Reference type: Journal
Authors: Han YT, Ma JQ, Yue XF, Zhang J
Article Title: Preparation of lysozyme based molecularly imprinted polymers and its chromatographic evaluation.
Publication date: 2012
Journal: Journal of Shaanxi Normal University (Natural Science Edition)
Volume: 40
Issue: (4)
Page numbers: 46-50.
Alternative URL: http://www.xuebao.snnu.edu.cn/PDF/LK_104_10.pdf

Abstract: Lysozyme-based molecularly imprinted polymers (Lys-MIPs) were prepared with PGMA/EDMA beads as a carrier material, lysozyme as the template molecule, β-cyclodextrin and acrylamide as the double functional monomers, N,N'-methylenebisacrylamide as the crosslinker. The prepared Lys-MIPs were used as a new HPLC packing material, based on which the related study on its recognition was conducted through the frontier chromatography. The results showed that Lys-MIPs had specific recognition for lysozyme, which helped achieve the separation between lysozyme and three individual competitive proteins, including bovine serum albumin (BSA), ovalbumin (OB) and myoglobin (Myo).
Template and target information: protein, lysozyme, Lys


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