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Reference type: Journal
Authors: Xia ZW, Lin Z, Xiao Y, Wang L, Zheng JN, Yang HH, Chen GN
Article Title: Facile synthesis of polydopamine-coated molecularly imprinted silica nanoparticles for protein recognition and separation.
Publication date: 2013
Journal: Biosensors and Bioelectronics
Volume: 47
Page numbers: 120-126.
DOI: 10.1016/j.bios.2013.03.024
Alternative URL: http://www.sciencedirect.com/science/article/pii/S095656631300184X

Abstract: Surface imprinting over nanostructured matrices is an effective solution to overcome template removal and achieve high binding capacity. In this work, a facile method was developed for synthesis of polydopamine-coated molecularly imprinted silica nanoparticles (PDA-coated MIP silica NPs) based on self-polymerization of dopamine (DA) on the surface of silica NPs in the presence of template protein. Transmission electronic microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR) and thermogravimetric analysis (TGA) showed that PDA layers were successfully attached on the surface of silica NPs and the corresponding thickness was about 5 nm, which enabled the MIP silica NPs to have fast binding kinetics and high binding capacity. Under the aqueous media, the imprinted silica NPs showed much higher binding affinity toward template than non-imprinted (NIP) silica NPs. The protein recognition properties were examined by single-protein or competitive batch rebinding experiments and rebinding kinetics study, validating that the imprinted silica NPs have high selectivity for the template. The resultant BHb-MIP silica NPs could not only selectively separate BHb from the protein mixture, but also specifically deplete high-abundance BHb from cattle whole blood. In addition, the stability and regeneration were also investigated, which indicated that the imprinted silica NPs had excellent reusability
Template and target information: protein, bovine hemoglobin, BHb
Author keywords: dopamine, molecular imprinting, protein recognition, silica nanoparticles, separation


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