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Abstract: A molecularly imprinted polymer (MIP) was prepared by using layer-by-layer (LbL) assembly and molecule imprinting of lysozyme and carboxymethyl cellulose. Firstly, the positively charged lysozyme and negatively charged carboxymethyl cellulose were alternately deposited onto the surface of the carboxymethyl cellulose substrate. After reacted with ferric chloride and eluted by 5.0 mol/L sodium chloride solution, the molecularly imprinted polymer of lysozyme was prepared. The surface morphologies of MIP were characterized using scanning electron microscope (SEM). Compared to non-imprinted polymer (NIP), the roughness of MIP surface increased. The adsorption capacity and selectivity of MIP were also discussed. The imprinted polymer had 50% higher adsorption capacity toward the template protein than the NIP. The imprinted polymer also had a higher selectivity for the imprinted molecule. In addition, the imprinted polymer showed reusability after several adsorption-desorption cycles
Template and target information: protein, lysozyme
Author keywords: Carboxymethyl cellulose, Layer-by-layer assembly, lysozyme, Protein imprinted polymer