Abstract: A novel preparation method of molecularly imprinted microspheres was developed based on molecular imprinting technique in combination with modified suspension polymerization for selective solid-phase extraction (SPE) of florfenicol residues in milk. The molecularly imprinted microspheres were prepared using florfenicol as the template, methacrylic acid as organic functional monomer, ethylene glycol dimethacrylate as the cross-linking agent. The solid-phase extraction conditions were optimized, and the optimal loading, washing and eluting solution respectively were 5%, 10% and 70% methanol aqueous solution. Under the optimized conditions, the chloramphenicols residues in milk were determined by the SPE-HPLC method. The average recoveries of three chloramphenicol antibiotics spiked milk at 0.005, 0.05 and 0.20 mg kg-1 were in the range of 73.4-92.0% with the precision of 1.7-4.3%. The limits of detection and quantitation of the proposed method were in a range of 0.0063-0.0246 mg kg-1 and 0.0209-0.082 mg kg-1, respectively.
Template and target information: Florfenicol, FF, chloramphenicol, CAP, thiamphenicol, TAP
Author keywords: molecularly imprinted microspheres, Florfenicol, Solid-phase extraction, milk