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Reference type: Journal
Authors: Andac M, Galaev IY, Denizli A
Article Title: Molecularly imprinted poly(hydroxyethyl methacrylate) based cryogel for albumin depletion from human serum.
Publication date: 2013
Journal: Colloids and Surfaces B: Biointerfaces
Volume: 109
Page numbers: 259-265.
DOI: 10.1016/j.colsurfb.2013.03.054
Alternative URL: http://www.sciencedirect.com/science/article/pii/S0927776513002440

Abstract: Macroporous cryogels imprinted with human serum albumin (HSA) have been prepared by copolymerization of 2-hydroxyethyl methacrylate with a functional co-monomer of N-methacryloyl-l-phenylalanine. The cryogels were used for the depletion of HSA from human serum. HSA-imprinted cryogels were prepared with gel fraction yields up to 90%, and their chemical structure, morphology and porosity were characterized by FTIR-spectroscopy, scanning electron microscopy, swelling studies and flow dynamics. Selective binding experiments were performed in the presence of competitive proteins like human transferrin and myoglobin. Albumin-imprinted cryogel column was optimized for fast protein liquid chromatography. Sodium-dodecyl sulfate polyacrylamide gel electrophoresis was used to show the efficiency of albumin depletion
Template and target information: protein, human serum albumin, HSA
Author keywords: Albumin depletion, Cryogels, protein imprinting, Proteome applications


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