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Abstract: A novel molecularly imprinted polymer (MIP) powder designed for the selective removal of interfering chlorophyll pigment from heavily pigmented green plant extracts during pesticide residue analysis or analysis for bioactives in natural product research is reported. The polymer powder imprinted with chlorophyll was synthesized by copolymerizing the functional and cross-linking monomers, methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA), respectively. During batch rebinding experiments, the MIP effectively removed chlorophyll from neat chlorophyll standards, green medicinal plants, and spinach extracts with determined absorbance as high as 2.501 absorbance units (Au) to as low as 0.084 Au after the optimum quantity of the MIP (170 mg mL-1) was added as the adsorbent prior to instrumental analysis. The determined 0.084 Au absorbance was far below the absorbance of 2% chlorophyll (0.401 Au) regarded as the cut-off point for interfering chlorophyll. Thus the newly developed polymer presents itself as a suitable adsorbent for the selective removal of chlorophyll from heavily pigmented, chlorophyll containing extracts.
Template and target information: chlorophyll