MIPs logo MIPdatabase      MIP2018 Conference website
Custom Search
Reference type: Journal
Authors: Bosserdt M, Gajovic-Eichelman N, Scheller FW
Article Title: Modulation of direct electron transfer of cytochrome c by use of a molecularly imprinted thin film.
Publication date: 2013
Journal: Analytical and Bioanalytical Chemistry
Volume: 405
Issue: (20)
Page numbers: 6437-6444.
DOI: 10.1007/s00216-013-7009-8

Abstract: We describe the preparation of a molecularly imprinted polymer film (MIP) on top of a self-assembled monolayer (SAM) of mercaptoundecanoic acid (MUA) on gold, where the template cytochrome c (cyt c) participates in direct electron transfer (DET) with the underlying electrode. To enable DET, a non-conductive polymer film is electrodeposited from an aqueous solution of scopoletin and cyt c on to the surface of a gold electrode previously modified with MUA. The electroactive surface concentration of cyt c was 0.5 pmol cm-2. In the absence of the MUA layer, no cyt c DET was observed and the pseudo-peroxidatic activity of the scopoletin-entrapped protein, assessed via oxidation of Ampliflu red in the presence of hydrogen peroxide, was only 30 % of that for the MIP on MUA. This result indicates that electrostatic adsorption of cyt c by the MUA-SAM substantially increases the surface concentration of cyt c during the electrodeposition step, and is a prerequisite for the productive orientation required for DET. After template removal by treatment with sulfuric acid, rebinding of cyt c to the MUA-MIP-modified electrode occurred with an affinity constant of 100,000 mol-1 L, a value three times higher than that determined by use of fluorescence titration for the interaction between scopoletin and cyt c in solution. The DET of cyt c in the presence of myoglobin, lysozyme, and bovine serum albumin (BSA) reveals that the MIP layer suppresses the effect of competing proteins
Template and target information: protein, cytochrome c, cyt c
Author keywords: Cytochrome C, Molecularly imprinted polymer film, Mercaptoundecanoic acid, Direct electron transfer, Scopoletin (7-hydroxy-6-methoxycoumarin)


  Periodic table Soup - the chemical formula for soup soup mug  Mug featuring the name Maria spelled out in the single letter amino acid code  Meerkat somebody mention coffee mug

Molecules Special Issue call






Join the Society for Molecular Imprinting
Logo of the Society for Molecular Imprinting

New items RSS feed
new items RSS feed  View latest updates

Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent e-mail alerts.
Click here to go to the sign-up page.


Is your name elemental or peptidic? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay:
US listings   UK listings

Searching for Molecular Imprinting books on Amazon.com:
Molecular Imprinting
Imprinted Polymers
Polymer Chemistry
Organic Chemistry
Biosensors

Find what you need at Amazon.com

Lab supplies from Amazon