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Reference type: Journal
Authors: Chen Y, He XW, Mao J, Li WY, Zhang YK
Article Title: Preparation and application of hollow molecularly imprinted polymers with a super-high selectivity to the template protein.
Publication date: 2013
Journal: Journal of Separation Science
Volume: 36
Issue: (20)
Page numbers: 3449-3456.
DOI: 10.1002/jssc.201300709

Abstract: Protein-imprinted polymers with hollow cores that have a super-high imprinting factor were prepared by etching the core of the surface-imprinted polymers that used silica particles as the support. Lysozyme as template was modified onto the surface of silica particles by a covalent method, and after polymerization and the removal of template molecules, channels through the polymer layer were formed, which allowed a single-protein molecule to come into the hollow core and attach to the binding sites inside the polymer layer. The adsorption experiments demonstrated that the hollow imprinted polymers had an extremely high binding capacity and selectivity, and thus a super-high imprinting factor was obtained. The as-prepared imprinted polymers were used to separate the template lysozyme from egg white successfully, indicating its high selectivity and potential application in the field of separation of protein from real samples
Template and target information: protein, lysozyme
Author keywords: Etching, Hollow core, lysozyme, Molecularly imprinted polymers, Protein separation


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