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Abstract: A new molecularly imprinted polymer (MIP) electrochemical sensor was prepared by anchoring an imprinted insulating poly(o-phenylenediamine) film on a glassy carbon electrode (GCE) surface for the determination of acephate in aqueous solution. In this protocol, the selection of a functional monomer was performed computationally using the density functional theory method to evaluate the template-monomer interaction energy. The electrochemical properties of the MIP-GCE were characterized by cyclic voltammetry and alternating current impedance. Under optimal experimental conditions, the peak current of the MIP-GCE performed by differential pulse voltammetry was related to the concentration of acephate in the range from 5.0 x 10-7 to 1.0 x 10-4 mol L-1. The detection limit of 1.3 x 10-7 mol L-1 and the limit of quantitation of 4.5 x 1.0-7 mol L-1 were obtained. The prepared MIP-GCE showed high recognition, sensitivity to acephate, and it provides potential for monitoring acephate residues in foods and water samples
Template and target information: acephate