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Reference type: Journal
Authors: Tretjakov A, Syritski V, Reut J, Boroznjak R, Volobujeva O, Ípik A
Article Title: Surface molecularly imprinted polydopamine films for recognition of immunoglobulin G.
Publication date: 2013
Journal: Microchimica Acta
Volume: 180
Issue: (15-16)
Page numbers: 1433-1442.
DOI: 10.1007/s00604-013-1039-y
Alternative URL: https://www.researchgate.net/publication/257446628_Surface_molecularly_imprinted_polydopamine_films_for_recognition_of_immunoglobulin_G

Abstract: We have prepared a surface imprinted polymer (SIP) film for label-free recognition of immunoglobulin G (IgG). The IgG-SIPs were obtained by covalent immobilization of IgG via a cleavable covalent bond and a suitable spacer unit to a gold electrode, followed by electrodepostion of a nm-thin film of polydopamine (PDA). The IgG was then removed by destruction of the cleavable bond so that complementary binding sites were created on the surface of the film. IgG-SIPs with various thicknesses of the PDA films were compared with respect to their affinity to IgG using a quartz crystal microbalance combined with flow injection analysis. The films were also characterized by cyclic voltammetry and scanning electron microscopy. The IgG-SIPs with a film thickness of around 17 nm showed the most pronounced imprinting effect (IF 1.66) and a binding constant of 296 nM
Template and target information: protein, immunoglobulin G, IgG
Author keywords: Molecularly imprinted polymers, IgG, Polydopamine, EQCM, QCM, FIA, electrochemical polymerization


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