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Abstract: New water-compatible molecularly imprinted microspheres were synthesized by reversible addition-fragmentation chain transfer (RAFT) polymerization using 3-(2-carboxyethylsulfanylthiocarbonyl-sulfanyl) propionic acid as a hydrophilic chain-transfer agent, and employed as the sorbent of pipet tip molecularly imprinted solid-phase extraction (PT-MISPE) for rapid extraction and screening of ofloxacin, pefloxacin, norfloxacin, ciprofloxacin, and enrofloxacin in eggs. In comparison to conventional SPE methods, the presented PT-MISPE showed special selectivity, easy operation, and accessible device without expensive SPE apparatus. The presented PT-MISPE method combined advantages of dummy molecularly imprinted polymers and pipet tip solid-phase extraction. The presented method was linear over a calibration range of 25-2500 μg/kg with the limits of detections of 0.53-1.07 μg/kg. Good recoveries (89.1-102.5%) were achieved with relative standard deviations of 2.6-4.8%
Template and target information: dummy template, Pazufloxacin, PAZ, ofloxacin, OFL, pefloxacin, PEF, norfloxacin, NOR, ciprofloxacin, CIP, enrofloxacin, ENR
Author keywords: Dummy molecularly imprinted microspheres, RAFT polymerization, pipette tip solid-phase extraction, Fluoroquinolones, Egg