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Reference type: Journal
Authors: Lian ZR, Wang JT
Article Title: Molecularly imprinted polymers for selective extraction of crystal violet from natural seawater coupled with high-performance liquid chromatographic determination.
Publication date: 2014
Journal: Journal of Ocean University of China
Volume: 13
Issue: (2)
Page numbers: 236-242.

Abstract: Molecularly imprinted polymers (MIPs) were prepared by the bulk polymerization using crystal violet as the template molecule, and the methacrylic acid and ethylene glycol dimetheacrylate as functional monomer and cross-linker, respectively. Systematic investigations of synthetic conditions were conducted. The surface morphology and recognition mechanism of the obtained polymers were studied using scanning electron microscope and spectrophotometric analysis. MIPs showed high affinity to template molecule and were successfully applied as special solid-phase extraction sorbent for selective extraction of crystal violet from natural seawater. An off-line molecularly imprinted solid-phase extraction (MISPE) method followed by high-performance liquid chromatography with diodearray detection for the analysis of crystal violet was also established. MISPE columns have good recoveries for crystal violet standard solutions and good linearity was obtained over the concentration range of 0-200 μg L-1 (R 2 > 0.99). Finally, two natural seawater samples were investigated. The recoveries of spiked seawater on the MISPE columns were from 44.47% to 62.34%, the relative standard deviation (n=3) being in the range of 2.89%-5.96%
Template and target information: crystal violet, CV, Basic Violet 3, Gentian violet
Author keywords: Crystal violet, molecularly imprinted polymer, adsorption, Solid-phase extraction, natural seawater

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