Inventors: Levon K
, Yu B
, Zhou YX
Publication date: 2005
A real-time, portable peptide-containing potentiometric biosensor that can directly identify bacterial spores. Two peptides for specific regognition of B. subtilis and B. anthracis Sterne may be immobilized by a polysiloxane monolyer immobilization (PMI) technique. The sensors translate the biological recognition even into a potential change by detecting, for example, B. subtilis spores in a concentration range 0.08-7.3x104 CFU/ml. The sensor exhibited highly selective recognition properties towards Bacillus subtilis spores over other kinds of spores. The selectively coefficients of the sensors for other kinds of spores are in the range of 0-1.0X10-5. The biosensor system not only has the specificity to distinguish Bacillus subtilis spores in a mixture of B. subtilis and B. thuringiensis (thur.) Kurstaki spores, but also can discriminate between live and dead B. subtilis spores. Furthermore, the sensor can distinguish a Bacillus subtilis 1A700 from other B. subtilis strain. Assay time may be as low as about 5 minutes for a single test. Rapid identification of B. anthracis Sterne and B. anthracis ?Ames was also provided.
Join the Society for Molecular Imprinting
New items RSS feed
View latest updates
Sign-up for e-mail updates:
Choose between receiving an occasional newsletter or more frequent
to go to the sign-up page.
Is your name elemental
? Enter your name and find out by clicking either of the buttons below!
Other products you may like:
view listings for MIP books on eBay: