Inventors: Chai CY, Li F, Wang L, Liu GY, Li WQ
Patent Title: Sensing electrode for detecting chloramphenicol and method for producing molecular imprinting film thereof
Publication date: 2009
Publication number: CN101424650

Abstract: The invention relates to a conductive type sensing electrode for detecting chloramphenicol in the field of biosensors and a method for preparing a molecular engram membrane of the chloramphenicol. In the invention, the conductive type sensing electrode is formed by arranging two screen painting electrodes in parallel. The method for preparing the molecular engram membrane of the chloramphenicol comprises the following steps: template molecules are embedded in a direct in-situ polymerization process of polymer fluid on the surfaces of the disposable screen painting electrodes, and then the template molecules are eluted so that cavities with set sizes and shapes and functional groups in confirmed arrangement are formed in the molecular engram membrane and have memorability to stereoscopic structures of the template molecules. Chloramphenicol molecules in a sample to be detected can be combined with the specificity of the cavities so as to change the electrochemical properties of the molecular engram membrane, and increase the conductivity and the membrane conductance of the molecular engram membrane. The processing method of the invention has simplicity, convenience and low cost, and the conductive type sensing electrode made of the screen painting electrodes has convenient operation, high response time and long service life when used for detecting the chloramphenicol.
URL: http://v3.espacenet.com/publicationDetails/biblio?DB=EPODOC&adjacent=true&locale=en_gb&FT=D&date=20090506&CC=CN&NR=101424650A&KC=A