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Inventors: Zhang DB, Qu GR, Wu AB
Patent Title: Method for preparing molecular imprinting polymer capable of identifying oxytetracycline and enrofloxacin
Publication date: 2009
Publication number: CN101397355

Abstract: The invention relates to a preparation method of a molecularly imprinted polymer which can distinguish terramycin and enrofloxacin base in the biochemistry engineering technology field. The method has the following steps: terramycin, enrofloxacin base, metallic ions, methacrylic acid and ethylene glycol dimethacrylate are mixed according to the mol ratio of 0.2-1: 0.6-1:1:2-10:20, and then dissolved in a porosity-making agent and added with an evocating agent azodiisobutyronitrile; the mixed liquor is placed in an ampere bottle and charged with nitrogen; the mouth of the ampere bottle is sealed under the protection of nitrogen; the water bath heat preservation reaction is carried out; after the synthetic polymer is stirred and scattered, kernels with the grain diameter of 0.1-1.5 Mum are obtained and subtle kernels are removed; the Soxhlet extraction is carried out; and the polymer which is gotten rid of template molecules is dried and stays the night, and the molecularly imprinted polymer is obtained. The polymer prepared by the method can be applied to the detection of residual terramycin and enrofloxacin base in the high water content environment or organism samples, and the enrichment of trace analytes and the removal of the matrix.
URL: http://v3.espacenet.com/publicationDetails/biblio?DB=EPODOC&adjacent=true&locale=en_gb&FT=D&date=20090401&CC=CN&NR=101397355A&KC=A

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