Inventors: Huang JD, Yu JH, Pei MS, Wang SH, Zhang YL, Wang YX, Zhang J, Ge SG, Li F, Zhang LN, Zhu YN, Mei Y, Lu Y, Li DM, Sun DZ, Xi ZF
Patent Title: Production method of molecular engram membrane electrode for detecting endocrine interference in trace amount surroundings and detecting method thereof
Publication date: 2008
Publication number: CN101294926

Abstract: The invention discloses a method for detecting an electrode of a molecular imprinting membrane of EDCs and the EDCs; the preparing method of the molecular imprinting membrane electrode comprises the following steps: a functional monomer is selected; template molecule of the EDCs, the functional monomer, a crosslinker, a porosity-making agent, an initiating agent and organic solvent are mixed evenly according to a certain molar ratio so as to prepare MIPs solution; nanometer material solution is prepared; the nanometer material and molecular imprinting polymer MIPs are modified on the surface of the electrode of a sensor by using the electrode surface modifying technology; a method for detecting trace amount of the EDCs comprises the following steps: the electrode of the molecular imprinting membrane is connected with an electrochemical working station and detects the EDCs in the environment sample extract; the electrode of the molecular imprinting membrane of the invention has strong specificity and high sensitivity which can reach ng level; a basic detection process is completed by only 1 to 2 minutes; the cost is low and the detection of one sample needs only a plurality of cents; the method for detecting the secretion in the environment by the electrode of the molecular imprinting membrane has fast and simple operation and the reaction and result are completed and recorded automatically by instruments.
URL: http://v3.espacenet.com/publicationDetails/biblio?DB=EPODOC&adjacent=true&locale=en_gb&FT=D&date=20081029&CC=CN&NR=101294926A&KC=A