Inventors: Zhang LH, Deng QL, Liu JX, Qiao XQ, Liang Z, Zhao P, Zhang YK
Patent Title: Production method for protein molecule imprinting integral column
Publication date: 2009
Publication number: CN101464439

Abstract: The invention relates to a method for preparing a protein molecular engram block polymer. Functional monomers, crosslinking agents and model protein molecules in proper proportions stand for 2 to 5 minutes at the temperature ranging from 0 to 4 DEG C, and a certain amount of evocating agents and rate accelerating materials are orderly added later. The mixture is put under the environment whose temperature ranges from minus 5 DEG C to minus 20 DEG C after being rapidly injected into a liquid chromatogram column jacket and polyreaction is completed within 1 to 12 hours. The model protein molecules are eluted by dodecyl sodium sulfate whose weight concentration is 10 percent and acetic acid solution whose volume concentration is 10 percent, namely, a macroporous protein molecular engram monolithic column can be prepared. The invention has the advantages as follows: the preparation method is simple and reliable, and the obtained protein molecular engram monolithic column has stronger template molecule recognition property. The technique provides a novel preparation method for developing protein molecular engram monolithic column.
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