Inventors: Zhang DB
, Wu AB
, Shi XZ
Method for preparing polymer microballons of molecular engram of nitrogen benzyl penicillin
Publication date: 2007
This invention discloses a method for preparing ampicillin molecularly imprinted polymer microspheres. The method comprises: preparing 3-6% poly(vinyl alcohol) solution, mixing functional monomers, template molecules, initiator, pore-foaming agent and crosslinker, ultrasonicating, adding into poly(vinyl alcohol) 1788 solution, stirring, stirring in 70 deg.C water for 60 min, filtering, ultrasonicating, washing with double distilled water twice, methanol three times and acetone twice (10 min each time), removing the template molecules, and vacuum-drying at 50 deg.C to obtain ampicillin molecularly imprinted polymer microspheres. The method has such advantages as simple process and high efficiency, and can prepare ampicillin molecularly imprinted polymer microspheres with high molecular recognition ability. When used in solid phase extraction, the ampicillin molecularly imprinted polymer microspheres have such advantages as high column efficiency and high selectivity. The ampicillin molecularly imprinted polymer microspheres can be used for selective separation and high-efficiency enrichment of penicillin in food matrix, such as honey.
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