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Inventors: Levi R, Margalit I, Dloomy Y
Patent Title: Method for preparing molecularly imprinted polymers and uses thereof
Publication date: 2011
Publication number: WO/2011/010304

Abstract: Methods for preparation of molecularly imprinted polymers and their use for detection of proteins and/or polypeptides in a sample are disclosed. The methods of preparation are based on selecting from available data bases an amino acid sequence of a protein/polypeptide target molecule; cleaving the sequence in-silico with at least one cleaving agent, producing fragments with known composition; selecting at least one such fragment comprising a unique epitope; preparing a synthetic peptide representing the unique epitope; and preparing a molecularly imprinted polymer comprising specific binding sites for the synthetic peptide. For detection of the target protein in a sample, the same cleaving agent used for the in-silico cleavage is used to cleave the target protein to form the specific peptide fragments to which the MIP is specific.
URL: http://v3.espacenet.com/textdoc?DB=EPODOC&IDX=WO2011010304&F=0

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