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Inventors: Ibii T, Hatakeyama S
Patent Title: Method for screening ligand
Publication date: 2011
Publication number: US20110212842

Abstract: The present invention provides a method for screening for a ligand having an affinity for a target substance and having readiness for conformational change forming a desired conformation upon binding to a target substance. The method includes the steps of (a) contacting a first mixture of candidate ligands with a carrier, followed by separating and collecting, as a second mixture of candidate ligands, a mixture of free candidate ligands not bound to the carrier, (b) contacting the second mixture of candidate ligands with the target substance, and (c) contacting the carrier with a solution containing the target substance and the mixture of candidate ligands obtained in step (b), and then separating and enriching a ligand, at least a part of which forms the particular conformation.
URL: http://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20110901&DB=EPODOC&locale=en_gb&CC=US&NR=2011212842A1&KC=A1

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