Inventors: Li WY, Zhang W, He XW, Zhang YK
Patent Title: Preparation method of lysozyme molecular imprinting-quantum dot nanoscale fluorescent probe
Publication date: 2012
Publication number: CN102313725

Abstract: The invention discloses a preparation method of a lysozyme molecular imprinting-quantum dot nanoscale fluorescent probe. The preparation method adopts quantum dots as carriers and lysozyme as a template molecule and modifies a molecular imprinting polymer layer on surfaces of the quantum dots to synthesize the novel lysozyme molecular imprinting-quantum dot nanoscale fluorescent probe through combining selectivity of a molecular imprinting technology and fluorescent characteristics of the quantum dots. Specially, the preparation method comprises the following steps of preparing quantum dots, modifying surfaces of the quantum dots by denatured bovine serum albumin, forming a molecular imprinting layer through a polymerization reaction initiated by a selected functional monomer and cross-linking reagents, and washing away the template molecule by selected eluent. The lysozyme molecular imprinting-quantum dot nanoscale fluorescent probe can combine accuracy and specific identification characteristics of molecular imprinting and high sensitivity characteristics of quantum dot fluorescence detection and has high selectivity and sensitivity of template molecule identification. The preparation method has simple processes, good reproducibility between different batches, good application prospects in selective identification and detection of lysozyme in an actual sample.
URL: http://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120111&DB=EPODOC&locale=en_EP&CC=CN&NR=102313725A&KC=A&ND=4