Inventors: Liu Z
, Li L
Molecularly imprinted polymer specially combined with specified glycoprotein and preparation method and application of molecularly imprinted polymer
Publication date: 2012
The invention discloses a molecularly imprinted polymer specially combined with a specified glycoprotein. Substituted phenylboronic acid containing double bonds is used as a functional monomer. Under the alkali condition, the specified glycoprotein serving as a template (imprinting molecule) and the substituted phenylboronic acid (functional monomer) containing the double bonds form a complex; the complex is mixed with a cross linker, an initiator and a pore forming agent; copolymerization reaction between the functional monomer and the cross linker and in the cross linker is initiated under the irradiation of ultraviolet light to form a polymer; and the template modules (specified glycoprotein) in the polymer are removed by extraction of an acidic solution, and thus the molecularly imprinted polymer containing a phenylboronic acid site reversibly combined with the glycosyl of the glycoprotein and a cavity complementary with the shape of the template modules is obtained. The molecularly imprinted polymer specially combined with the specified glycoprotein has the advantages of high specificity, strong anti-interference capacity, wide practical sample range, good repeatability, simplicity in preparation and the like, and can be directly used for detecting antigens to be detected in a complex biological system of serum, saliva and the like. The invention discloses a preparation method for the molecularly imprinted polymer.
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