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Inventors: Lin Z, Lin Y, Yang HH, Zhang L, Chen GN
Patent Title: Organic-inorganic hybrid protein molecular engram capillary tube monolithic column
Publication date: 2012
Publication number: CN102626609

Abstract: The invention discloses an organic-inorganic hybrid protein molecular engram capillary tube monolithic column and a preparation method and an application thereof. The preparation method comprises the following steps of: mixing a silane precursor, a dispersant and a catalyst, and hydrolyzing; adding into a mixed solution containing an organic functional monomer, a crosslinking agent, a pore-foaming agent, an initiator and template proteins; performing ultrasonic treatment at the temperature of 0-4 DEG C to a full dissolving state; injecting into a vinyl-modified quartz capillary tube; and performing an in-situ polymerization reaction to obtain the organic-inorganic hybrid protein molecular engram capillary tube monolithic column. The organic-inorganic hybrid protein molecular engram capillary tube monolithic column is easy to operate, has mild reaction conditions, and has the advantages of an organic monolithic column and an inorganic monolithic column; and the specific identification characteristic of a molecular engram is combined with the online separation characteristic of the monolithic column, and a novel method for removing abundant proteins is provided for the research of proteomics.
URL: http://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120808&DB=EPODOC&locale=en_EP&CC=CN&NR=102626609A&KC=A&ND=1

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