Inventors: Liu N, Gao ZX, Ma XH, Ou GR, Li XL, Chen X
Patent Title: Two-dimensional molecular engram array type quality sensing detection method for staphylococcus enterotoxin
Publication date: 2012
Publication number: CN102565161

Abstract: The invention discloses a two-dimensional molecular engram array type quality sensing detection method for staphylococcus enterotoxin, which comprises the following steps: (1) performing surface active treatment on a quartz oscillating electrode; (2) preparing an initial sol-gel; (3) preparing an engram sol-gel of a template containing SEA (staphylococcus enterotoxin A) and SEB (staphylococcus enterotoxin B); (4) performing coating treatment on the surface of the quartz oscillating electrode; (5) removing protein molecules SEA and SEB of the template; and (6) constructing a molecular engram array quality sensor for the SEA and SEB detection. The conventional SEA and SEB macromolecular proteins in the staphylococcus enterotoxin which is the important virulence factor of sitotoxismus are quickly detected. The method is simple; no mark is required; the detection speed is high; the external interference is small; the cost is low; the result is stable and reliable; the time of the whole detecting process is short; and the method has a wide application prospect in the fields of food safety, hygiene detection, environment monitoring, clinical examination, biological terror monitoring, and the like.
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