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Inventors: Levi R, Margalit I, Laub O, Dloomy Y
Patent Title: Method for preparing protein imprinted polymers and use thereof
Publication date: 2013
Publication number: US20130137117

Abstract: Methods for preparation of molecularly imprinted polymers and their use for detection of proteins and/or polypeptides in a sample are disclosed. The methods of preparation are based on selecting from available data bases an amino acid sequence of a protein/polypeptide target molecule; cleaving the sequence in-silico with at least one cleaving agent, producing fragments with known composition; selecting at least one such fragment comprising a unique epitope; preparing a synthetic peptide representing the unique epitope; and preparing a molecularly imprinted polymer comprising specific binding sites for the synthetic peptide. For detection of the target protein in a sample, the same cleaving agent used for the in-silico cleavage is used to cleave the target protein to form the specific peptide fragments to which the MIP is specific.
URL: http://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20130530&DB=EPODOC&locale=en_EP&CC=US&NR=2013137117A1&KC=A1&ND=4

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