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Inventors: Zhang L, Lu QM, Fan LB, Cai HJ, Chen GN
Patent Title: Preparation method and application of indole acetic acid (IAA) molecularly imprinted polymer (MIP)
Publication date: 2012
Publication number: CN102702565

Abstract: The invention belongs to the field of analytical chemistry and particularly relates to a preparation method and application of indole acetic acid (IAA) molecularly imprinted polymer (MIP). The preparation method and the application are suitable for enriching and analyzing growth hormone plant samples. According to the method, IAA is used as template molecules, a functional monomer, a crosslinking agent and initiator are added so as to obtain a prepolymerization solution; and reaction is performed at the temperature of 60 DEG C for more than 8 hours, and the template modules are removed so as to obtain the IAAMIP. The IAAMIP can be used for a solid-phase micro-extraction (SPME) coating. The treated quartz fiber is inserted in the prepolymerization solution and is subjected to thermal-initiation polymerization; after reaction, the quartz fiber is taken out, aged and coated repeatedly; the template modules are cleaned completely; and finally the fiber is inserted into a micro-extraction device for subsequent extraction. The IAA-MIP and SPME (MISPME) coating is easy to prepare, controllable in thickness, high in selectivity and good in enrichment effect, and can be used repeatedly. An extraction head is combined with liquid chromatogram, so that two kinds of growth hormone in the plant samples can be efficiently extracted and separated.
URL: http://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20121003&DB=EPODOC&locale=en_EP&CC=CN&NR=102702565A&KC=A&ND=4

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