Inventors: Li JP
, Chang C
, Wei XP
Method for measuring gibberellin in fruits by aid of molecularly imprinted electrochemically modified electrode
Publication date: 2012
The invention discloses a method for measuring gibberellin in fruits by the aid of a molecularly imprinted electrochemically modified electrode. The gibberellin and methylene blue are mixed and are electrochemically polymerized to form a membrane on the surface of a glassy carbon electrode, and a molecularly imprinted electrochemical sensor is prepared after the gibberellin is eluted. Along with the increase of the quantity of the gibberellin which is marked by dopamine on various recognition sites on the molecular imprinted membrane and is eluted, current of oxidation peaks is greatly reduced, and accordingly the electrochemical analysis method for measuring the trace gibberellin is built. Good linear relation between peak current I and the gibberellin within the concentration range from 2.5X10-8 to 1.05X10-6mol/L is good. The method overcomes various shortcomings such as excessive complexity in the prior art, is high in measuring sensitivity, wide in measuring linear range and low in detection limit, and has an excellent effect in detecting the gibberellin with the concentration of 10-8mol/L in the fruits.
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