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Inventors: Li ZZ, Xu H, Li WR, Li DM, Hou YZ, Zhang M, Li ZK, Liu SL
Patent Title: Preparation method of halofuginone molecularly-imprinted solid-phase extraction small column and application
Publication date: 2013
Publication number: CN102539587

Abstract: The invention provides a preparation method of a halofuginone molecularly-imprinted solid-phase extraction small column and an application and relates to the field of analytical chemistry. The preparation method comprises the following steps of: taking halofuginone as a template molecule, dissolving the template molecule into a porogen and adding a functional monomer at a low temperature for prepolymerization; then, mixing according to the molar ratio of the template molecule: the functional monomer: a cross-linking agent of 1:3-6:15-30, and preparing molecularly-imprinted polymer micro-spheres in a manner of initiated polymerization at a low temperate by adopting a suspension polymerization method and filling the rinsed, sieved, eluted and dried micro-spheres in an empty column tube of the solid-phase extraction column by adopting a wet process; and later, using the micro-spheres rinsed by acetone and methanol in sequence into selective adsorption, enrichment and purification of the halofuginone remained in animal derived foods. Compared with the existing solid-phase extraction column, the halofuginone molecularly-imprinted solid-phase extraction column prepared by the invention has the characteristics of strong specificity, convenience and simplicity in preparation, good recognition performance, low cost, easiness for realization of required instruments and reaction conditions and the like.
URL: http://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20120704&DB=EPODOC&locale=en_EP&CC=CN&NR=102539587A&KC=A&ND=4

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