Inventors: Li ZZ, Li DM, Hou YZ, Du L, Xu H, Zhang M, Liu SL, Li ZK, Li WR
Patent Title: Preparation method and application of phloroglucinol molecularly imprinted polymers in plants
Publication date: 2012
Publication number: CN102580693

Abstract: The invention relates to a preparation method and application of phloroglucinol molecularly imprinted polymers in plants. The method comprises the following steps of: mixing template molecules, a functional monomer and a cross-linking agent in a molar ratio of 1:(3-6):(15-30), and adding benzoyl peroxide and N,N-dimethylaniline which serve as redox initiators; dripping the mixture into a 3 to 8 percent polyvinyl alcohol 400 aqueous solution with stirring at the temperature of between 10 and 30 DEG C to obtain polymer microspheres; bleaching the polymer microspheres, sieving, eluting, drying and the like, and filling in a glass chromatographic column by a wet method; and moistening the polymer microspheres by using methanol and acetone sequentially to obtain a molecularly imprinted chromatographic column, so that the molecularly imprinted chromatographic column is used for the preferential adsorption and efficient enrichment of phloroglucinol substances such as hyperforin, adhyperforin and the like. Compared with the conventional chromatographic column, the prepared chromatographic column has the characteristics that the column is high in specificity, easy and convenient to prepare and low in cost, required instruments and reaction conditions are easy to implement, and the like.
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