Inventors: Chen LG
, Wang T
, Tong J
Method for detecting antibiotic residue in soil by using matrix solid-phase dispersion technique
Publication date: 2011
The invention discloses a method for detecting antibiotic residue in soil by using a matrix solid-phase dispersion technique, relates to a method for detecting antibiotic residue in soil, and aims to solve the problems of complex sample pretreatment process, time consumption and low selectivity in the conventional method for detecting the antibiotic residue in the soil. The method comprises the following steps of: 1, preparing molecularly imprinted polymer; 2, grinding a soil sample and the molecularly imprinted polymer and uniformly mixing; 3, transferring the mixture of the soil sample and the molecularly imprinted polymer into an extraction column and leaching with a leaching agent; 4, draining and eluting to obtain eluent; 5, blowing the eluent by using nitrogen gas to obtain residue; and 6, dissolving the residue, filtering and performing chromatographic or chromatographic tandem mass spectrographic analysis to finish detection. By adopting the method, the pretreatment step in the detection is simplified, the time is saved, the consumption of an organic solvent is reduced, and the selectivity of a detection and analysis method is greatly improved. The method is applied to the field of detection of antibiotic residue.
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