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Inventors: Cui LF, Zhao S, Shen Q
Patent Title: Preparation and application of melamine molecular imprinting electrochemical sensor
Publication date: 2011
Publication number: CN101942086

Abstract: The invention relates to a molecular imprinting electrochemical sensor, which is used for determining melamine in liquid milk and belongs to the technical field of food safety detection. The melamine in the liquid milk is taken as an object of study, and a molecularly imprinted polymer is prepared by electropolymerization on a gold electrode by using the cyclic voltammetry method. The invention constructs a new method for selectively detecting the melamine by taking mixed solution of equimolar hydroxyphenol and o-aminophenol as functional monomers, taking the melamine as template molecules and taking K3Fe(CN)6 as a probe between an imprinting electrode and base solution. The reaction conditions are respectively as follows: the monomer/template mass ratio is 0.55/1, the pH value is 5.5, and the temperature is 30 DEG C. The molecularly imprinted polymer imprinted polymer thin film which leaves template molecular configuration holes is prepared by using methanol solution to carry out ultrasonic elution for 6min and removing the template molecules from the electrode. The detection is carried out through the differential pulse method, and the linear range is 0.50-7.50mg/kg. The response time is 10 seconds. The molecular imprinting electrochemical sensor can be used for detecting an actual liquid milk sample without pretreatment, the selectivity is good, and the recovery rate of adding standard is 87.5-109%.
URL: http://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20110112&DB=EPODOC&locale=en_EP&CC=CN&NR=101942086A&KC=A&ND=4

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