Inventors: Zhang LH
, Liu JX
, Deng QL
, Liang Z
, Zhang YK
Method for preparing protein molecularly imprinted polymer particles
Publication date: 2011
The invention relates to preparation of protein molecularly imprinted polymer particles, in particular relates to a particulate organic polymer synthesized by a protein molecularly imprinted technology. The protein molecularly imprinted polymer particles are prepared through the following steps: preparing a reacting solution; adsorbing a protein solution in the ducts of porous silica gel by oscillation or filtration and other methods; pumping the reacting solution into the ducts of the silica gel through a vacuum pump or other methods; performing a polymerization or agglomeration reaction in the ducts of the silica gel; and corroding silicon spheres after the polymerization or agglomeration reaction is completed so as to obtain the protein molecularly imprinted polymer particles.; The invention has the advantages that the preparation method is simple and convenient, and the polymer particles have regular particle diameters and large adsorption capacity to template protein. Compared with other molecularly imprinted polymer particle preparation methods, the method provided by the invention has few preparation steps, and the prepared polymer particles have the characteristics of high adsorption and desorption speed on the template protein as well as good specificity identification to the template protein.
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