Inventors: Jiang FY
, Li JP
, Wei XP
Method for determining trace terramycin by utilizing molecularly imprinted immunosensor
Publication date: 2010
The invention discloses a method for determining trace terramycin by utilizing a molecularly imprinted immunosensor. When an terramycin molecularly imprinted membrane is formed on the surface of a gold electrode and acts with terramycin molecules, an electrochemical signal of hydroquinone on the gold electrode changes; and when hydrogen peroxide and horseradish peroxidase exist in a detection system, the electrochemical signal has more remarkable change. When terramycin in solution to be measured is utilized to competitively replace original horseradish peroxidase labeled terramycin on the molecularly imprinted membrane, the electrochemical signal can be linearly reduced along with the increase of the concentration of unlabeled terramycin so as to establish an electrochemical analysis method for determining the trace terramycin. A differential pulse voltammetry is used to scan the solution to be measured, the scanning voltage is between -0.6V and 0.8V, and the terramycin is in good linear relation with a peak current value i within the concentration range of between 0 and 8*10-8mol/L. The method overcomes the disadvantages of high complexity and the like existing in the prior art; and the detection limit of the terramycin reaches 10-9mol/L.
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