Inventors: Huang JD, Yu JH, Pei MS, Wang SH, Ge SG, Lin Q, Zhang XM, Xing XR, He XR, Zhu H, Sun NX, Song XY, Yuan L
Patent Title: Method for preparing glycosyl functional molecularly imprinted membrane electrode for detecting bacterial toxin and application thereof
Publication date: 2010
Publication number: CN101666774

Abstract: The invention relates to a method for preparing a glycosyl functional molecularly imprinted membrane electrode for detecting bacterial toxin, which comprises the following steps: (1) selecting a functional monomer which can compound a glycosyl functional molecularly imprinted polymer with the bacterial toxin; (2) uniformly mixing a bacterial toxin template molecule, the functional monomer, a cross-linking agent, a porogenic agent, an initiating agent and an organic solvent to prepare a glycosyl functional molecularly imprinted polymer solution according to a certain molar ratio; and (3) modifying the glycosyl functional molecularly imprinted polymer on the surface of an electrode of a sensor by an electrode surface modifying technology.; The method for detecting trace bacterial toxin comprises the following steps: connecting the glycosyl functional molecularly imprinted membrane electrode prepared by the method to an electrochemical workstation and detecting the bacterial toxin in an environmental sample extracting solution. The glycosyl functional bacterial toxin molecularly imprinted membrane electrode has high specificity, sensitivity and detecting sped, can realize high flux screening of a plurality of samples in short time and reduces the detecting cost.
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