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Inventors: Huang JD, Wang SH, Yu JH, Pei MS, Ge SG, Zhang XM, Xing XR, He XR, Lin Q, Zhu H, Sun NX, Song XY, Yuan L
Patent Title: Glycosyl functional bacterial toxin molecularly imprinted membrane substrate as well as preparation method and application thereof
Publication date: 2010
Publication number: CN101666773

Abstract: The invention relates to a glycosyl functional bacterial toxin molecularly imprinted membrane substrate as well as a preparation method and an application thereof. The molecularly imprinted membrane substrate forms a reaction layer on the surface of a gold quartz crystal substrate by taking a glycosyl functional bacterial toxin molecularly imprinted polymer as a recognition element. The preparation method comprises the following steps: selecting a carbohydrate molecule functional monomer which can carry out specificity recognition with bacterial toxin and compound the corresponding molecularlyimprinted polymer; preparing a glycosyl functional molecularly imprinted polymer solution; and modifying the glycosyl functional molecularly imprinted polymer on the surface of the gold quartz crystal substrate by a substrate surface modifying technology to form a reaction layer.; The glycosyl functional molecularly imprinted membrane substrate prepared by the method is connected to a piezoelectric quartz crystal microbalance to detect bacterial toxin in an environmental sample extracting solution. The molecularly imprinted membrane substrate has favorable molecular recognition performance andgreatly improves the sensitivity and the selectivity for detecting the bacterial toxin.
URL: http://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20100310&DB=EPODOC&locale=en_EP&CC=CN&NR=101666773A&KC=A&ND=4

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