Inventors: Huang JD, Yu JH, Wang SH, Pei MS, Ge SG, Xing XR, He XR, Lin Q, Zhang XM, Zhu H, Sun NX, Song XY, Yuan L
Patent Title: Method for preparing glycosyl functional bacterial toxin molecularly imprinted column and application thereof
Publication date: 2010
Publication number: CN101666752

Abstract: The invention relates to a method for preparing a glycosyl functional bacterial toxin molecularly imprinted column, which comprises the following steps: selecting a carbohydrate functional monomer which can compound a glycosyl functional molecularly imprinted polymer with bacterial toxin; preparing a glycosyl functional molecularly imprinted polymer solution; and modifying the glycosyl functionalmolecularly imprinted polymer on the inner surface of a glass pipe column by a surface modifying technology. In the method for detecting bacterial toxin, the glycosyl functional molecularly imprintedcolumn prepared by the method is connected to a chemical luminescent analyzer, and a chemical luminescent system solution and the sample solution are respectively pumped into the chemical luminescentanalyzer to detect the bacterial toxin in a sample.; The prepared glycosyl functional bacterial toxin molecularly imprinted column has high specificity, sensitivity and detecting sped, can realize highflux screening of a plurality of samples in short time and reduces the detecting cost.
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