Inventors: Zhang YQ
Konjac glucomannan stroma protein molecularly imprinted membrane as well as preparation method and application thereof
Publication date: 2011
The invention provides a protein molecularly imprinted membrane which can selectively distinguish combined template protein molecules and takes konjac glucomannan as a main matrix. The preparation method comprises the following steps: fully swelling the konjac glucomannan, and adding cross-linking agent or mixing with other macromolecule; adding template protein molecules after neutralizing; fully mixing and salivating to form a membrane; drying and dehydrating to form molecular imprinting of the template protein; adding eluent; and fully eluting the template protein molecules to prepare into the konjac glucomannan stroma protein molecularly imprinted membrane.; The protein molecularly imprinted membrane of the invention can be used as a biological separation simulating affinity membrane to be applied to protein separation and purification, especially the separation and the purification of genetic engineering protein products, and also can be used as sensitive membrane components which are in biosensing detection and are used for simulating antibodies to identify the corresponding antigens and the like.
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