Inventors: Zhang SW, Wu CY, Jun X, Cai LS
Patent Title: Molecular imprinting monolithic column, preparation method and application thereof
Publication date: 2010
Publication number: CN101690886

Abstract: The invention discloses a molecular imprinting monolithic column, a preparation method and application thereof. The molecular imprinting monolithic column comprises a quartz capillary tube and a molecularly imprinted polymer in the quartz capillary tube. Guanosine is used as template molecules. The method comprises the following steps:; Guanosine, acrylamide, 4-vinyl pyridine, N,N'-methylene-bisacrylamide and dodecyl alcohol are dissolved in dimethyl suloxide by a water-bath method and an ultrasonic technique at the temperature of 40-50 DEG C, and deoxidized, then azodiisobutyronitrile is added into the dissolved solution to mix uniformly, wherein the mass ratio of guanosine, acrylamide, 4-vinyl pyridine, N,N'-methylene-bisacrylamide, dodecyl alcohol, dimethyl suloxide and azodiisobutyronitrile is 17.5:55-58:48-56:62-64:275-285:350-360:1.8-2.2, and at last polymerization is carried out at a constant temperature of 55-60 DEG C for 16-20 hours in the quartz capillary tube modified by 3-(triethoxylsilane) propyl metacrylic acid ester or 3-(trimethylsiyl) propyl metacrylic acid ester to prepare the finished product.; The molecular imprinting monolithic column is used for extracting 8-hydroxyl desoxyguanosine and guanosine with higher beneficiation ability and selectivity.
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