Inventors: Zhang LH, Deng QL, Liu JX, Ma JF, Liang Z, Zhang YK
Patent Title: Protein imprinted material and use thereof for removing albumin from human blood serum
Publication date: 2009
Publication number: CN101463105

Abstract: The invention relates to a preparation method of a pig serum albumin molecularly imprinted material and application thereof to selectively removing the albumin from human serum. The method comprises the following steps: the pig serum albumin is taken as a template; a protein imprinted material is obtained by an in situ polyreaction of a functional monomer, a cross-linking agent, a pore-forming agent, a surfactant and template protein, and the characteristic that the human serum albumin has high homology with the pig serum albumin is employed to successfully applying the material to removing the albumin from the human serum. The method can help solve the problem of difficult albumin sources by taking human albumin as imprinted templates, and realize the selective removal of the albumin from the human serum by the prepared molecularly imprinted material.; The invention provides a new high-abundance protein removal method for proteomics studies.
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